Induction of robust cytotoxic T lymphocytes using adenovirus-vectored multivalent antigen cocktail

• DOI: 10.4049/jimmunol.196.supp.216.17
• Publication Date: 2023-01-01T10:44:38Z
• Authors: Waithaka Mwangi; Shehnaz Lokhandwala; Surya Waghela
• Keywords: Immunogen; CTL*; Priming (agriculture)

Abstract Development of subunit vaccines against complex pathogens entails inclusion multiple targets to improve efficacy and or broaden protection. Importantly, testing an immunogen in the actual host that require protection can hasten vaccine development generate knowledge needed other similar vaccines. The African Swine Fever Virus (ASFV) causes a fatal hemorrhagic disease domestic swine and, at present, no treatment is available. Previous studies suggest induction ASFV-specific cytotoxic T lymphocytes (CTLs) critical for We evaluated immunogenicity adenovirus-vectored ASFV multivalent cocktail formulated two adjuvants doses swine. induced unprecedented CTL responses all antigens cocktail. Notably, were detected immunized with low dose (1 × 1010 ifu/each construct) high 1011 construct). In addition, both elicited strong responses. Significant antigen-specific IFN-γ+ PBMCs vaccinees post-priming post-boosting. Furthermore, robust antibody underwent rapid isotype-switching within one week post-priming. primed recall upon boost four months Taken together, this study showed first time safely cellular following homologous prime-boost immunization. relevance immune regards will be challenge study.