Sequence-specific detection of different strains LCMV in a single sample using tentacle probes

• DOI: 10.4049/jimmunol.196.supp.69.3
• Publication Date: 2023-01-01T10:16:04Z
• Authors: Lina S Franco; Susan A Holechek; Michael Caplan; Joseph N Blattman
• Keywords: clone (Java method); Strain (injury); Arenavirus

Abstract Viral infections often result in quasi-species of virus strains that can have dramatic impacts on disease outcomes or experimental results. However, sequencing viruses to determine strain composition is time consuming and cost-prohibitive. Rapid, cost-effective methods are needed for accurate measurement diversity order design appropriate treatment, be useful many systems. We developed a novel molecular method simple detection RNA genetic variants using Tentacle probes coupled with quantitative PCR. modified beacons less expensive dramatically improve false positive rates routine qPCR. To validate this approach we designed two tentacle Lymphocytic Choriomeningitis Virus (LCMV): the parental Armstrong more virulent clone-13 strain, which differ by only 3 nucleotide substitutions. Using were able distinguish these LCMV. Furthermore, mixing experiments detect down 5% diluted Armstrong, vice versa. Thus, fast, differentiating between closely related viruses.