Dendritic Cells Require TMEM176A/B Ion Channels for Optimal MHC Class II Antigen Presentation to Naive CD4+ T Cells

• DOI: 10.4049/jimmunol.2000498
• Publication Date: 2021-07-07T17:56:38Z
• Authors: Melanie Lancien; Geraldine Bienvenu; Sonia Salle; Lucile Gueno; Magalie Feyeux; Emmanuel Merieau; Severine Remy; Amandine Even; Aurelie Moreau; Alice Molle; Cynthia Fourgeux; Flora Coulon; Gaelle Beriou; Laurence Bouchet-...; Elise Chiffoleau; Peggy Kirstetter; Susan Chan; Steven M Kerfoot; Saeed Abdu Rahiman; Veronica De Simone; Gianluca Matteoli; Gaelle Boncompain; Franck Perez; Regis Josien; Jeremie Poschmann; Maria Cristina Cu...; Cedric Louvet
• Keywords: Priming (agriculture); Antigen processing; CD1D; CD1

Intracellular ion fluxes emerge as critical actors of immunoregulation but still remain poorly explored. In this study, we investigated the role redundant cation channels TMEM176A and TMEM176B (TMEM176A/B) in retinoic acid-related orphan receptor γt+ cells conventional dendritic (DCs) using germline conditional double knockout mice. Although Tmem176a/b appeared surprisingly dispensable for protective function Th17 group 3 innate lymphoid intestinal mucosa, found that they were required DCs optimal Ag processing presentation to CD4+ T cells. Using a real-time imaging method, show TMEM176A/B accumulate dynamic post-Golgi vesicles preferentially linked late endolysosomal system strongly colocalize with HLA-DM. Taken together, our results suggest play direct MHC class II compartment fine regulation naive cell priming.