Circular RNA AEBP2 modulates dendritic cells by the hnRNP/pP65 signaling pathway

• DOI: 10.4049/jimmunol.204.supp.164.24
• Publication Date: 2023-01-01T14:11:43Z
• Authors: Qinfeng Zhou; Bowen Wang; James Koropatnick; Weiping Min; Vivian McAlister; Adam Greasley; Xiufen Zheng
• Keywords: CD80; Heterogeneous nuclear ribonucleoprotein; Circular RNA

Abstract Circular RNA (circRNA) has been the focus of research in recent years. However, circRNA is rarely reported dendritic cells (DCs). In our study, Microarray analysis showed that circular AEBP2 (circAEBP2) significantly up-regulated mature DCs compared with immature DCs. Knockdown circAEBP2 DC siRNA arrests at status lower expression MHC class II, CD40, CD80, and CD83, as well decreasing Phosphorylation nuclear factor-kappa B p65. CircAEBP2 -silenced failed to activate allogeneic CD4+ T CD8+ vitro. immunoprecipitation assays show heterogeneous ribonucleoproteins (hnRNP) are enriched by probes which have complementary sequence, indicating interacts hnRNP. The effect silenced on induction anti-tumor immunity investigated an ongoing murine melanoma model. conclusion, results demonstrate regulates development function through interacting hnRNP/pP65 signaling pathway. will be a new potential target for DCs-based immunotherapy.