Single-Cell Analysis Reveals the Heterogeneity of Monocyte-Derived and Peripheral Type-2 Conventional Dendritic Cells

• DOI: 10.4049/jimmunol.2100094
• Publication Date: 2021-07-19T20:05:21Z
• Authors: Yuehan Gao; He Li; Zhaohuai Li; Lihui Xie; Xiuxing Liu; Zhaohao Huang; Binyao Chen; Xianchai Lin; Xianggui Wang; Yingfeng Zheng; Wenru Su
• Keywords: Monocyte

Dendritic cells (DCs) are critical for pathogen recognition and Ag processing/presentation. Human monocyte-derived DCs (moDCs) have been extensively used in experimental studies DC-based immunotherapy approaches. However, the extent of human moDC peripheral heterogeneity their interrelationship remain elusive. In this study, we performed single-cell RNA sequencing moDCs blood DCs. We identified seven subtypes within moDCs: five corresponded to type 2 conventional (cDC2s), other two were CLEC10A+CD127+ with no resemblance any DC subpopulations characterized date. Moreover, defined similar cDC2s, revealed potential differentiation trajectory among them, unveiled transcriptomic differences between cDC2s. further studied changes each subtype during maturation, demonstrating SLAMF7 IL15RA as maturation markers CLEC10A SIGLEC10 immature These findings will enable more accurate functional/developmental analyses cDC2s moDCs.