Mucosal CCL28 Chemokine Improves Protection against Genital Herpes through Mobilization of Antiviral Effector Memory CCR10+CD44+ CD62L−CD8+ T Cells and Memory CCR10+B220+CD27+ B Cells into the Infected Vaginal Mucosa

• DOI: 10.4049/jimmunol.2300093
• Publication Date: 2023-05-24T12:27:49Z
• Authors: Nisha Rajeswari D...; Swayam Prakash; Afshana Quadiri; Latifa Zayou; Ruchi Srivastava; Jennifer Tran; Vivian Dang; Amin Mohammed Shaik; Amruth Chilukurri; Berfin Suzer; Phil Vera; Miyo Sun; Pauline Nguyen; Ashley Lee; Amirah Salem; Joyce Loi; Mahmoud Singer; Takashi Nakayama; Hawa Vahed; Anthony B Nesburn; Lbachir BenMohamed
• Keywords: CCR10; CCL20; CCL5; CC chemokine receptors

Abstract Four major mucosal-associated chemokines, CCL25, CCL28, CXCL14, and CXCL17, play an important role in protecting mucosal surfaces from infectious pathogens. However, their protection against genital herpes remains to be fully explored. The CCL28 is a chemoattractant for the CCR10 receptor–expressing immune cells produced homeostatically human vaginal mucosa (VM). In this study, we investigated of CCL28/CCR10 chemokine axis mobilizing protective antiviral B T cell subsets into VM site infection. We report significant increase frequencies HSV-specific memory CCR10+CD44+CD8+ cells, expressing high levels CCR10, herpes-infected asymptomatic (ASYMP) women compared with symptomatic women. Similarly, (a ligand CCR10), was detected ASYMP C57BL/6 mice, associated mobilization effector CCR10+CD44+CD62L−CD8+ TEM CCR10+B220+CD27+ HSV-infected mice. Inversely, wild-type knockout (CCL28−/−) mice (1) appeared more susceptible intravaginal infection reinfection HSV type 2, (2) exhibited decrease CD27+B220+ infected VM. These findings suggest critical within protect disease.