ANTIMICROBIAL ACTIVITY OF EXTRACTS, ESSENTIAL OIL AND METABOLITES OBTAINED FROM TAGETES MENDOCINA

• DOI: 10.4067/s0717-97072009000100016
• Publication Date: 2009-07-31T14:51:35Z
• Authors: BEATRIZ LIMA; MARÍA BELÉN AGÜERO; JULIO ZYGADLO; ALEJANDRO TAPIA; CARLOS SOLIS; ANTONIETA ROJAS D...; GLORIA YALUFF; SUSANA ZACCHINO; GABRIELA EGLY FER...; GUILLERMO SCHMEDA...
• Keywords: Tagetes

Extracts from the Argentinean herb Tagetes mendocina Phil.(Asteraceae) were tested for antimicrobial activity against fungi, bacteria and protozoa.The different extracts (PE, DCM, MeOH EtOAc) essential oil displayed mainly dermatophytes, a group of fungi that produces skin infections.The bioassay-guided fractionation PE, DCM EtOAc led to isolation eight compounds including two thiophenes, acetophenone derivatives, dihydrorosefuran three flavonoids.The thiophenes 5-(4-hydroxy-1-butynyl)-2,2 ' -bithienyl (BBTOH) (1) its acetate (BBTOAc) (2) proved be highly active dermatophytes Microsporum gypseum, Trichophyton rubrum T. mentagrophytes with MIC values 3.12, 3.12 6.25 µg/mL compound 1 25, 2, respectively.In turn, weak moderate antifungal effect M. gypseum = 250 µg/mL.The was examined by GC GC-MS 21 constituents (97.3%) identified.(E) β-ocimene, (Z)-tagetone, (E)-tagetone, (Z)-ocimenone, α-pinene (E)-ocimenone major components.In addition, 2 2-hydroxyacetophenone 3 showed antiprotozoal Leishmania amazonensis, L. brasiliensis infantum promastigotes 100% lysis at 100 µg/mL.