Coagulase-negative staphylococci causing blood stream infection at an Indian tertiary care hospital: Prevalence, antimicrobial resistance and molecular characterisation

• DOI: 10.4103/0255-0857.195374
• Publication Date: 2017-01-25T05:05:21Z
• Authors: S Singh; B Dhawan; A Kapil; SK Kabra; A Suri; V Sreenivas; BK Das
• Keywords: cons; Coagulase; SCCmec; Staphylococcus haemolyticus; Teicoplanin

Introduction: Recent years have seen a rise of coagulase-negative staphylococci (CoNS) from common contaminants to agents nosocomial blood stream infections (BSI’s). Molecular typing and establishing correlation with antibiotic resistance is essential particularly in countries like India where genotyping studies for drug-resistant CoNS are sparse. Methods: A prospective study was done over 18 months, wherein 42,693 samples were received, 59 patients BSI due evaluated. The isolates recovered identified by biochemical test panel matrix-assisted laser desorption ionization – time flight mass spectrometry followed antimicrobial susceptibility testing Kirby–Baur disc diffusion method E-test strips. Staphylococcal chromosomal cassette mec (SCCmec) element characterised multiplex polymerase chain reaction all methicillin-resistant (MR) isolates. Results: majority isolated constituted Staphylococcus haemolyticus (47.5%) epidermidis (33.9%), hominis (11.86%), cohnii (5.08%) warneri (1.69%). Among 57.6% MR statistically significant higher versus methicillin sensitive-CoNS. This difference erythromycin (76% vs. 44%, P = 0.011), rifampicin (50% 12%, 0.002) amikacin (26.5% 4%, 0.023), ciprofloxacin (64.7% 20%, 0.001) cotrimoxazole (55.9% 0.006). SCCmec type I predominant (61.8%, 0.028) exhibited multidrug (76.2%). Coexistence III 8.82% Conclusion: exhibit high thereby limiting treatment options. presence new variants hospital-acquired may predict the pattern. first evaluation molecular epidemiology causing can serve as guide formulation hospital infection control guidelines.