Promoting effect of triterpenoid compound from Agrimonia pilosa Ledeb on preadipocytes differentiation via up-regulation of PPARγ expression

• DOI: 10.4103/0973-1296.149741
• Publication Date: 2015-01-20T12:33:36Z
• Authors: Liancai Zhu; Bochu Wang; Tingwang Guo; Jun Tan; Xuemei Zhou; Ling Xiao; Xi Liu
• Keywords: Oil Red O

Pharmacognosy Magazine,2015,11,41,219-225.DOI:10.4103/0973-1296.149741Published:January 2015Type:Original ArticleAuthors:Tingwang Guo, Liancai Zhu, Jun Tan, Xuemei Zhou, Ling Xiao, Xi Liu, and Bochu Wang Author(s) affiliations:Tingwang Guo1, Zhu1, Tan2, Zhou1, Xiao1, Liu1, Wang1 1 Key Laboratory of Biorheological Science Technology, Ministry Education, College Bioengineering, Chongqing University, Chongqing, China 2 School Biological Chemical Engineering, University Abstract:Background: Agrimonia Pilosa Ledeb (APL), a traditional Chinese medicine, has been reported variety biological activities, including treating T2DM. Objective: Triterpenoid compound (TC) was collected from APL. The aim this study to investigate the effects TC on 3T3-L1 preadipocytes differentiation genes related IR. Materials Methods: Column chromatography used collect ALP. cell induced typically in presence various concentrations or pioglitazone. Oil red O staining measurement intracellular TG content were performed seventh day differentiation. Then quantitative polymerase chain reaction (Q-PCR) test expressions three transcription factors (PPARγ, CCAAT enhancer binding protein-α (C/EBP-α), sterol regulatory element-binding protein (SREBP-1)) target PPARγ glucose transporter (GLUT4), lipoprotein lipase (LPL), fat acid (AP2), adiponectin cells. Results: At concentration 5, 25 125 μg/mL, significantly promoted triglyceride accumulation. Further showed that could promote expression PPARγ, C/EBPα ADD1/SREBP1 at μg/mL. As for downstream controlled by μg/mL GLUT4 adiponectin. However, aP2 lipid metabolism adiposity group lower than pioglitazone group. Conclusion: through activating genes. ideal insulin sensitization with adipogenic action classical TZDs vitro. So good prospect as natural drug IR Keywords:Agrimonia Ledeb, aP2, triterpenoid compound, type diabetes mellitusView:PDF (1.92 MB) Full Text