Baseline effects of lysophosphatidylcholine and nerve growth factor in a rat model of sciatic nerve regeneration after crush injury

• DOI: 10.4103/1673-5374.232479
• Publication Date: 2018-05-29T13:31:41Z
• Authors: AlonzoD Cook; RyanL Wood; KeatonS Karlinsey; AustinD Thompson; MarkN Rigby; GreggoryD Boatright; WilliamG Pitt; BeverlyL Roeder; ScottC Steffensen
• Keywords: Crush injury

Schwann cells play a major role in helping heal injured nerves. They help clear debris, produce neurotrophins, upregulate neurotrophin receptors, and form bands of Büngner to guide the healing nerve. But nerves do not always enough neurotrophins receptors repair themselves. Nerve growth factor (NGF) is an important for promoting nerve lysophosphatidylcholine (LPC) has been shown stimulate NGF (NGFR). This study tested administration single intraneural injection LPC (1 mg/mL 10 multiple injections) at day 0 one (day 7), two (days 5 or three 5, 7, 9) injections (160 ng/mL 80 determine baseline effects on crushed sciatic rats. The rats were randomly divided into four groups: control, crush, crush-NGF, crush-LPC-NGF. was measured weekly by monitoring gait; electrophysiological parameters: compound muscle action potential (CMAP) amplitudes; morphological total fascicle areas, myelinated fiber counts, densities, packing, mean g-ratio values weeks 3 6. crush-LPC-NGF groups statistically differed from control group all six parameters but only week parameters. did differ each other over course study. Single apart treatments 7 9 days post-injury alter rate during 1-6 These findings are define injections, as part larger effort minimal dose regimen regenerate peripheral