The effects of fibrin–icariin nanoparticle loaded in poly (lactic-co-glycolic) acid scaffold as a localized delivery system on chondrogenesis of human adipose-derived stem cells

• DOI: 10.4103/abr.abr_143_19
• Publication Date: 2020-02-25T08:51:10Z
• Authors: Batool Hashemibeni; Mona Gorji; Nazem Ghasemi; Mohsen Setayeshmehr; Anooshe Zargar; Mohammad Kazemi; Mitra Soleimani
• Keywords: PLGA; Chondrogenesis; Viability assay; Icariin; MTT assay

Nowadays, cartilage tissue engineering is the best candidate for regeneration of defects. This study evaluates effect fibrin/icariin (ICA) nanoparticles (F/I NPs) on chondrogenesis stem cells.F/I NPs were characterized by Dynamic Light Scattering DLS. Poly (lactic-co-glycolic) acid (PLGA)-F/I NP scaffold was fabricated and assessed scanning electron microscope. Human adipose-derived cells (hADSCs) seeded induced chondrogenesis. After 14 days, cell viability gene expression analyzed 3-(4, 5- dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. MTT assay real-time polymerase chain reaction (RT-PCR).The size surface charge F/I about 28-30 nm - 17, respectively. The average pore PLGA PLGA-fibrin/ICA 230 340 μm, Cell differentiated in P/F group higher than others significantly (P ≤ 0.05). Furthermore, quantitative RT-PCR analysis demonstrated that ICA upregulated cartilaginous-specific expression. results type I collagen revealed downregulated this < 0.01).The indicated could be a potential factor downregulation fibrocartilage marker.