Normative data for paediatric lymphocyte subsets: A pilot study from western India

• DOI: 10.4103/ijmr.ijmr_3282_21
• Publication Date: 2024-04-16T07:00:24Z
• Authors: Neha Jodhawat; Umair Ahmed Bargir; Priyanka Setia; Prasad Taur; Nidhi Bala; Aditi Madkaikar; Reetika Malik Yadav; Aparna Dalvi; Shweta Shinde; Maya Gupta; Shraddha Shelar; Priyanka Kambli; Vijaya Gowri; Madhukar Lokeshwar; Purnima Satoskar; Mukesh Desai; Manisha Madkaikar
• Keywords: Cord blood; Reference values

Accurate diagnosis of immunodeficiencies requires a critical comparison values with age-matched controls. In India, the existing reference for rare lymphocyte subsets are currently not available and we rely on data originating from other countries interpretation results. Furthermore, there is limited information normal variation these rare-subset parameters in Indian children. So, this study aimed to establish normative clinically important children at different age groups.148 aged ≥16 yr were enrolled study. The population included 61 per cent males 39 females was divided into following groups: cord blood (n=18), 0-6 months (n=9), 6-12 (n=13), 1-2 (n=19), 2-5 (n=27), 5-10 (n=25) 10-16 (n=37). absolute relative percentage lymphocytes, T, B, natural killer cell, along activated, naïve memory subsets, determined by flow cytometry.Median 10th 90th percentiles obtained 34 sub-populations. T B compartments showed decreasing trend, whereas cells an increase age. activated cell subset shows increasing pattern up one year then declines gradually. Double negative relatively stable. TCRgd+T increases age.This single-centre pilot provides preliminary that justifies need future large-scale multi centric studies generate range interpreting extended immunophenotyping profiles paediatric group, making it possible clinicians assess immunological status inborn errors immunity, infectious autoimmune diseases.