Identification of Drought-Responsive Universal Stress Proteins in Viridiplantae

• DOI: 10.4137/bbi.s6061
• Publication Date: 2011-02-07T20:31:21Z
• Authors: Raphael D. Isokpehi; Shaneka S. Simmons; Hari H.P. Cohly; Stephen I.N. Ekunwe; Gregorio B. Begonia; Wellington K. Ayensu
• Keywords: UniProt

Genes encoding proteins that contain the universal stress protein (USP) domain are known to provide bacteria, archaea, fungi, protozoa, and plants with ability respond a plethora of environmental stresses. Specifically in plants, drought tolerance is desirable phenotype. However, limited focused organized functional genomic datasets exist on drought-responsive plant USP genes facilitate their characterization. The overall objective investigation was identify diverse Expressed Sequence Tags (ESTs) responsive water-deficit stress. We hypothesize cross-database mining annotations gene transcript bioinformatics resources would help candidate transcripts from multiple species. Our approach retrieved, mined integrated comprehensive annotation data 511 1561 ESTs sequences 161 viridiplantae taxa. A total 32 7 genera Glycine, Hordeum, Manihot, Medicago, Oryza, Pinus Triticum were identified. Two Arabidopsis At3g62550 At3g53990 encode ATP-binding motif up-regulated microarray dataset. Further, dataset 80 simple sequence repeats (SSRs) linked 20 singletons 47 assembles constructed. Integrating SSRs identified three bread wheat (BE604157), soybean (BM887317) maritime pine (BX682209). SSR types CAG, ATA AT respectively. for characterization as useful targets engineering varieties tolerant unfavorable conditions.