Improved rat spinal cord injury model using spinal cord compression by percutaneous method

• DOI: 10.4142/jvs.2013.14.3.329
• Publication Date: 2013-09-28T06:37:45Z
• Authors: Wook-Hun Chung; Jae-Hoon Lee; Dai-Jung Chung; Wo-Jong Yang; A-Jin Lee; Chi-Bong Choi; Hwa-Seok Chang; Dae-Hyun Kim; Hyo Jin Chung; Hyun Jung Suh; Soo-Han Hwang; Hoon Han; Sun Hee Do; Hwi-Yool Kim
• Keywords: Spinal cord compression; Lumbosacral joint; Epidural space; Balloon catheter

Here, percutaneous spinal cord injury (SCI) methods using a balloon catheter in adult rats are described. A was inserted into the epidural space through lumbosacral junction and then inflated between T9-T10 for 10 min under fluoroscopic guidance. Animals were divided three groups with respect to inflation volume: 20 μL (n = 18), 50 18) control (Fogarty but not inflated; n 10). Neurological assessments made based on BBB score, magnetic resonance imaging histopathology. Both volumes produced complete paralysis. Gradual recovery of motor function occurred when used, after applied. In group, all gray white matter lost from center lesion. addition, supramaximal damage noted, which likely prevented spontaneous recovery. This compression model is simple, rapid high reproducibility potential serve as useful tool investigation pathophysiology possible protective treatments SCI vivo.