Whole genome sequencing analysis of enteropathogenic Escherichia coli from human and companion animals in Korea

• DOI: 10.4142/jvs.24225
• Publication Date: 2024-12-12T06:58:48Z
• Authors: Jae Young Oh; Kyung-Hyo Do; Jae Hong Jeong; SuMin Kwak; Sujin Choe; Dongheui An; Jong-Chan Chae; Kwangjun Lee; Kwang-Won Seo
• Keywords: Enteropathogenic Escherichia coli

This study is essential for comprehending the zoonotic transmission, antimicrobial resistance, and genetic diversity of enteropathogenic Escherichia coli (EPEC). To improve our understanding EPEC, this focused on analyzing comparing genomic characteristics EPEC isolates from humans companion animals in Korea. The whole genome 26 patients with diarrhea 20 Korea were sequenced using Illumina HiSeq X (Illumina, USA) Oxford Nanopore MinION (Oxford Technologies, UK) platforms. Most atypical did not harbor bfpA gene. most prevalent virulence genes found to be ompT (humans: 61.5%; animals: 60.0%) followed by lpfA 46.2%; 60.0%). Although pan-genome analyses showed no apparent correlation among origin strains, profiles, resistance included clade A obtained both exhibited high similarity. Additionally, all encoded gene encode hlyE two harbored an incomplete bundle-forming pilus region encoding bfpB. Moreover, type IV secretion system-associated tra trb bfpA-encoding humans. Whole-genome sequencing enabled a more accurate analysis phylogenetic structure provided better insights into epidemiology pathogenicity.