Cigarette smoking extract causes hypermethylation and inactivation of WWOX gene in T-24 human bladder cancer cells

• DOI: 10.4149/neo_2012_028
• Publication Date: 2011-12-09T06:00:57Z
• Authors: W. YANG; S. CUI; J. MA; Q. LU; C. KONG; T. LIU; Z. SUN
• Keywords: WWOX; FHIT; Chromosomal fragile site

Genomic, epigenetic and expression alterations of WW domain containing oxidoreductase (WWOX) have been implicated in multiple tumor types. The current study was designed to examine the WWOX tissues human bladder transitional cell carcinoma (BTCC) influence cigarette smoke extract (CSE) on methylation status T-24 cancer cells. protein evaluated by immunohistochemistry staining a series samples from 78 patients with BTCC 26 normal tissues. level CSE-treated cells were examined using quantitative Real-Time RT-PCR specific PCR, respectively. levels DNA methyltransferases (DNMTs) 1, 3A 3B also detected. We found that absent or reduced 79.5% (62/78) tissues, but only 19.2% (5/26) Loss correlated grade (P=0.019) smoking (P=0.031), not associated age, gender, size number. Hypermethylation promoter exon 1 specifically induced CSE kinetics concurrent suppression mRNA Furthermore, treatment significant time-dependent increase DNMT1, has no effects DNMT3A DNMT3B. Taken together, these novel findings suggest hypermethylation may represent one underlying mechanism for loss cancer.