Dental pulp mesenchymal stem/stromal cells labeled with iron sucrose release exosomes and cells applied intra-nasally migrate to intracerebral glioblastoma

• DOI: 10.4149/neo_2016_611
• Publication Date: 2016-08-27T05:59:00Z
• Authors: U. ALTANEROVA; K. BENEJOVA; V. ALTANEROVA; S. TYCIAKOVA; B. RYCHLY; P. SZOMOLANYI; F. CIAMPOR; M. CIHOVA; V. REPISKA; K. ONDICOVA; B. MRAVEC; C. ALTANER
• Keywords: Ferric Oxide, Saccharated; 0301 basic medicine; 03 medical and health sciences; Cell Movement; Humans; Mesenchymal Stem Cells; Exosomes; Glioblastoma; Administration, Intranasal; Dental Pulp; Cell Proliferation

We report on a simple iron oxide (Venofer) labeling procedure of dental pulp mesenchymal stem cells (DP-MSCs) and DP-MSCs transduced with yeast cytosinedeaminase::uracilphosphoribosyltransferase (yCD::UPRT-DP-MSCs). Venofer is drug approved for intravenous application to treat deficiency anemia in patients. did not affect or yCD::UPRT-DP-MSCs viability growth kinetics. Electron microscopy labeled showed internalized nanoparticles endosomes. MRI relativity measurement phantom arrangement revealed that 100 per 0.1 ml were still detectable. the corresponding release exosomes into conditional medium (CM). CM from presence prodrug 5-fluorocytosine caused tumor cell death dose dependent manner. Iron sustained their tropism vivo; intra-nasally applied migrated specifically engrafted orthotopic glioblastoma xenografts rats.