Tetramethylpyrazine (TMPZ) triggers S-phase arrest and mitochondria- dependent apoptosis in lung cancer cells

• DOI: 10.4149/neo_2018_170112n26
• Publication Date: 2018-05-17T09:04:16Z
• Authors: H. H. HUANG; F. B. LIU; Z. RUAN; J. ZHENG; Y. J. SU; J. WANG
• Keywords: Propidium iodide; Tetramethylpyrazine

Tetramethylpyrazine (TMPZ) is one of the active compounds extracted from traditional Chinese herb Chuanxiong.Several studies have shown its anti-cancer properties.However, functions in lung cancer and underlying cellular mechanisms are relatively unknown.Our present study aimed to investigate effects TMPZ on A549 95D cells.The MTT assay showed that decreased cell viability a dose-and time-dependent manner.The results colony formation indicated strongly suppressed ability cells.Flow cytometric analysis revealed induced S phase arrest cells.In addition, apoptosis, as by propidium iodide/Annexin V double-staining.Furthermore, mitochondrial membrane potential (∆Ψm) dose-dependent manner.Finally, western blot TMPZ-treated cells activation Caspase-3 increase ratio Bax/Bcl-2.These demonstrate can suppress carcinogenesis through blocking cycle inducing mitochondria-dependent apoptosis regulating Bax/Bcl-2, suggesting may be promising drug treat cancer.