MiR-374b targets GATA3 to promote progression and development of glioblastoma via regulating SEMA3B

• DOI: 10.4149/neo_2018_180830n659
• Publication Date: 2019-03-14T09:32:37Z
• Authors: J. Gao; S. Bai; Y. Wang; S. Zhao; Z. He; R. Wang
• Keywords: GATA3; Viability assay

In the present study, a series of experiments were conducted to explore function miR-374b and regulatory relationship among miR-374b, GATA3 SEMA3B in glioma.MiR-374b mimics inhibitors employed regulate expression.Besides, qRT-PCR assay was used for detecting expression level mRNAs.To verify targeting between GATA3, dual luciferase analysis utilized.Moreover, chromatin immunoprecipitation (ChIP) performed identify correlation with SEMA3B.Furthermore, si1-GATA3, si2-GATA3 pc-GATA3 pc-SEMA3B served dysregulation SEMA3B.For assessing significance miR374b alone or co-operation on cell viability, migration apoptosis, CCK-8, transwell FCM also performed.We found that overexpression which identified glioma tissues lines (U251, LN-299 GOS-3) promoted enhanced viability but inhibited apoptosis this study.Furthermore, contributed increase decrease targeted by as evidenced assay.Moreover, binding promoter SEMA3B, involved regulating revealed.Further, studies demonstrated resulted elevation suppressed apoptosis.However, promotion effects miR-374 process reversed co-transfecting pc-SEMA3B.In conclusion, promotes vitro through suppressing via GATA3.The result study provides an important clue optimal treatment schedule glioblastoma.