Knockdown of lncRNA SNHG4 suppresses gastric cancer cell proliferation and metastasis by targeting miR-204-5p

• DOI: 10.4149/neo_2021_200914n981
• Publication Date: 2021-02-11T00:40:38Z
• Authors: Xian-Bin Cheng; Tao Zhang; Hong-Jing Zhu; Ning Ma; Xiao-Dan Sun; Shou-Han Wang; Yang Jiang
• Keywords: Gene Expression Regulation, Neoplastic; Mice; MicroRNAs; 0303 health sciences; 03 medical and health sciences; Stomach Neoplasms; Cell Line, Tumor; Animals; RNA, Long Noncoding; Cell Proliferation; 3. Good health

Long non-coding RNAs (lncRNAs) have been identified as critical regulators in gastric cancer (GC) progression.However, whether lncRNA small nucleolar host gene 4 (SNHG4) functions GC development remains unknown.In this study, the bio-functional role of SNHG4 and its potential mechanism on progression were systematically dissected.To investigate GC, we silenced using short hairpin (shRNAs) to perform loss-of-function assays.The results showed that expression cells was at a higher level compared normal mucosal epithelial cells.Knockdown dramatically suppressed proliferation, migration invasion, blocked cell cycle cells.Moreover, knockdown upregulated microRNA-204-5p (miR-204-5p) expression, whereas downregulated ribonucleotide reductase subunit M2 (RRM2) cells.Dual-luciferase reporter assay miR-204-5p direct target SNHG4.Additionally, tumorigenesis xenograft mouse models.Taken together, these data demonstrated by targeting miR-204-5p.