SNHG16 lncRNAs are overexpressed and may be oncogenic in human gastric cancer by regulating cell cycle progression

• DOI: 10.4149/neo_2021_210518n680
• Publication Date: 2021-12-06T07:59:56Z
• Authors: Juan-Juan Zhao; Juan-Juan Liu; Yun-Yuan Zhang; Ying Xia; Hong Du; Zhi-Qiang Yan; Chun-Huan Zhou; Wan-Song Xia; Lucas Zellmer; De-Zhong Joshua Liao; Si-Xi Wei; Hai Huang
• Keywords: Cell Cycle; Oncogenes; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; 0302 clinical medicine; Cell Movement; Stomach Neoplasms; Cell Line, Tumor; Humans; RNA, Long Noncoding; Cell Proliferation

The small nucleolar RNA host gene 16 (SNHG16) has recently been shown to be a putative oncogene in gastric cancer (GC) and other types, but how its four lncRNA variants are expressed any physiological pathological situation remains unknown. To investigate the expression function of SNHG16, mainly variant 1, GC, we performed quantitative PCR determine levels 60 GC tissue samples several cell lines. We also studied knocking down SNHG16 with siRNA affected proliferation, apoptosis, cycle progression, as well migration invasion cells. Our results showed that 1 4 were overexpressed tissues compared adjacent uninvolved tissues. Knockdown variants, enhanced apoptosis inhibited progression line by arresting cells at G1 phase. These cellular effects associated not only decreased protein c-Myc, PCNA, cyclins D1, E1, A2 B, CDKs 2 6, increased p21, p27 p53. total lncRNAs vitro. collectively suggest may oncogenic regulating serve biomarker.