Practical Applications of Integrated Microfluidics for Peptide Quantification

• DOI: 10.4155/bio.15.15
• Publication Date: 2015-05-01T16:03:09Z
• Authors: Erin E Chambers; Mary E Lame; Paul D Rainville; James Murphy; Jay Johnson; Kenneth J Fountain; Robert S Plumb; Peter Claise; Norman W Smith
• Keywords: Microscale chemistry; Bioanalysis

Increased pressure to obtain more, higher sensitivity data from less sample is especially critical for large peptides, whose already optimized LC-MS methods are heavily challenged by traditional ligand-binding assays.Critical bioanalytical assays were adapted integrated microscale LC reduce volumes while increasing sensitivity. Assays teriparatide, glucagon and human insulin five analogs transferred 2.1 mm analytical scale a 150 µm system. This resulted in 15-30 fold overall improvement derived increased signal noise, three six reduction injection volumes, two consumption.Integrated reduces consumption enabling single picomolar quantification therapeutic endogenous peptides.