CIAPIN1 inhibits gastric cancer cell proliferation and cell cycle progression by downregulating cyclyinD1 and upregulating p27

• DOI: 10.4161/cbt.6.10.4684
• Publication Date: 2010-06-04T18:15:36Z
• Authors: Xiaohua Li; Zhiming Hao; Rui Fan; Xue Zou; Haifeng Jin; Yanglin Pan; Lijie He; Rui Du; Lin Gao; Jie Liu; Daiming Fan
• Keywords: Ectopic expression

AbstractCIAPIN1, a newly identified apoptosis-related molecule, was found to be downregulated in human gastric cancer tissues as compared the matched adjacent nonneoplastic tissues. In this study, we investigated effect of CIAPIN1 on vitro and vivo proliferation cells. Ectopic expression knockdown cells SGC7901 MKN45 were achieved by transduction with recombinant adenoviruses expressing (Ad-CIAPIN1) CIAPIN1-specific small interference RNA (Ad-CIAPIN1siRNA). Gastric upregulated exhibited significantly decreased proliferation, while showed quicker growth rate their respective controls both tests. Also, suppressed colony formation soft agar cloning test. Furthermore, cell cycle distribution analysis revealed that induced arrest at G1/S phase. Downregulation CyclyinD1 upregulation P27 might contribute, least part, altered distribution. This study demonstrates is suppressor suggests play an important role development cancer.