p14ARF sensitizes human osteosarcoma cells to cisplatin-induced apoptosis in a p53-independent manner

• DOI: 10.4161/cbt.6.7.4324
• Publication Date: 2010-07-01T18:08:11Z
• Authors: Xiang-Wei Yuan; Xiao-Feng Zhu; Xiu-Fang Huang; Pu-Yi Sheng; Ai-Shan He; Zi-Bo Yang; Rong Deng; Gong-Kan Feng; Wei-Ming Liao
• Keywords: p14arf

AbstractThe tumor suppressor p14ARF, encoded by the INK4a/ARF locus, is often disrupted in human cancers. p14ARF triggers cell cycle arrest and sensitizes cells to apoptosis presence of collateral signals. To investigate role chemotherapeutic drugs-induced apoptosis, was overexpressed stable transfection osteosarcoma lines, U2OS (p53-wt/p14ARF-null) MG63 (p53-mt/p14ARF-null). The results showed that ectopic sensitized both lines cisplatin-induced cytotoxicity apoptosis. This sensitization associated with upregulation p53, Bax p21 cells. Conversely, such a result not observed Moreover, unaltered p53 siRNA. Together, we show here p53-independent manner. Proper combinations gene transfer conventional chemotherapy may be valuable strategy treatment.