Homeodomain protein Dlx3 induces phosphorylation-dependent p63 degradation

• DOI: 10.4161/cc.8.8.8202
• Publication Date: 2010-10-25T21:16:35Z
• Authors: Antonella Di Cost...; Luisa Festa; Olivier Duverger; Maria Vivo; Luisa Guerrini; Girolama La Mantia; Maria I. Morasso; Viola Calabrò
• Keywords: Protein Degradation

The epidermis is a stratified epithelium which develops depending on the transcription factor p63, member of p53 family factors. p63 strongly expressed in innermost basal layer where highly proliferative epithelial cells reside. functions as molecular switch that initiates stratification or cell fate determination while regulating proliferation and differentiation developmentally mature keratinocytes. acts upstream Dlx3 homeobox gene transcriptional regulatory pathway relevant to ectodermal dysplasia. Here we show triggers protein degradation by proteasome-dependent pathway. Mutant ΔNp63α Threonine397 Serine383 were replaced with Alanine well C-terminal truncated versions are resistant Dlx3-mediated degradation. Transient expression associated Raf1 phosphorylation. unable promote depleted MEF upon pharmacological knockdown Raf1. Our data support previously unrecognized role for posttranslational regulation level, mechanism may contribute reduce abundance during epithelia.