Trophoblast-specific DNA methylation occurs after the segregation of the trophectoderm and inner cell mass in the mouse periimplantation embryo

• DOI: 10.4161/epi.7.2.18962
• Publication Date: 2012-02-01T20:27:01Z
• Authors: Momo O. Nakanishi; Koji Hayakawa; Kazuhiko Nakabayashi; Kenichiro Hata; Kunio Shiota; Satoshi Tanaka
• Keywords: Inner cell mass; Differentially methylated regions; Trophoblast

The first cell differentiation in the mammalian development separates trophoblast and embryonic lineages, resulting formation of trophectoderm (TE) inner mass (ICM) blastocysts. Although a lower level global DNA methylation genome TE compared with ICM has been suggested, dynamics profile during TE/ICM not elucidated. To address this issue, we identified tissue-dependent differentially methylated regions (T-DMRs) between stem (TS) (ES) cells. Most these TS–ES T-DMRs were also tissues day (E) 6.5 mouse embryos. Furthermore, found that human genomic homologous to placental ES Collectively, defined them as cell-lineage-based lineages (T–E T-DMRs). Then, examined cells isolated from E3.5 Interestingly, all examined, including Elf5, Pou5f1 Nanog loci, nearly unmethylated status both exhibited no differences. present results suggest establishment profiles specific each lineage follows morphological specification. Together previous reports on asymmetry histone modifications ICM, current study imply function landmarks for setting up cell-lineage-specific differential profiles.