Gestational intake of methyl donors and global LINE-1 DNA methylation in maternal and cord blood: Prospective results from a folate-replete population

• DOI: 10.4161/epi.7.3.19082
• Publication Date: 2012-03-12T16:50:29Z
• Authors: Caroline E. Boeke; Andrea Baccarelli; Ken P. Kleinman; Heather H. Burris; Augusto A. Litonjua; Sheryl L. Rifas-S...; Letizia Tarantini; Matthew Gillman
• Keywords: Cord blood; Choline

Maternal diet affects offspring DNA methylation in animal models, but evidence from humans is limited. We investigated the extent to which gestational intake of methyl donor nutrients global maternal and umbilical cord blood. Among mother-infant pairs Project Viva, a folate-replete US population, we estimated intakes vitamin B12, betaine, choline, folate, cadmium, zinc iron periconceptionally during second trimester. examined associations these with methylation, measured as %5-methyl cytosines (%5mC) Long Interspersed Nuclear Element-1 (LINE-1), first trimester (n = 830) 671) blood at delivery 516). Cord was higher for male than female infants {mean [standard deviation (SD)] 84.8 [0.6] vs. 84.4 [0.7]%}. In multivariable-adjusted model, pregnancy not positively associated trimester, or LINE-1 methylation. Periconceptional betaine inversely [regression coefficient -0.08% (95% confidence interval (CI): -0.14,-0.01)] this association attenuated after adjustment dietary itself directly also found an inverse between periconceptional choline [-0.10%, 95% CI: -0.17,-0.03 each SD (~63 mg/d)] males only. did find positive overall, among males, early were lower