Further characterization ofShigella sonneilive vaccine candidates WRSs2 and WRSs3-plasmid composition, invasion assays and Sereny reactions

• DOI: 10.4161/gmic.2.4.17042
• Publication Date: 2011-10-14T16:41:58Z
• Authors: Lisa Bedford; Suramya Fonseka; Tara Boren; Ryan T. Ranallo; Akamol E. Suvarna...; James E. Lee; Shoshana Barnoy; Malabi M. Venkatesan
• Keywords: Shigella sonnei

Shigella sonnei live vaccine candidates WRSs2 and WRSs3 are principally attenuated by the loss of virG(icsA) gene that prevents bacterial spread. In addition both strains lack enterotoxin SenA a paralog SenB. also has deletion in encoding MsbB2. Both these expected to be improved safer versions WRSS1 only lacked was reactogenic volunteer trials. During construction WRSs3, segments virulence plasmid spanning two FRT sites were found inverted orientation. These inversions have been analysed show overall composition is unaltered. Furthermore, characterization cell culture an animal model conclusively demonstrated segment per se do not adversely affect behavior pose no increased safety risk for human evaluation.