Compound K attenuates stromal cell-derived growth factor 1 (SDF-1)-induced migration of C6 glioma cells

• DOI: 10.4162/nrp.2016.10.3.259
• Publication Date: 2016-05-25T06:45:44Z
• Authors: Hyuck Kim; Hyo Sun Roh; Jai Eun Kim; Sun Dong Park; Won Hwan Park; Jin-Young Moon
• Keywords: Viability assay

Stromal cell-derived growth factor 1 (SDF-1), also known as chemokine ligand 12, and receptor type 4 are involved in cancer cell migration. Compound K (CK), a metabolite of protopanaxadiol-type ginsenoside by gut microbiota, is reported to have therapeutic potential therapy. However, the inhibitory effect CK on SDF-1 pathway-induced migration glioma has not yet been established.Cytotoxicity C6 cells was determined using an EZ-Cytox viability assay kit. Cell tested wound healing Boyden chamber assay. Phosphorylation levels protein kinase C (PKC)α extracellular signal-regulated (ERK) were measured western blot assay, matrix metallopeptidases (MMP) gelatin-zymography analysis.CK significantly reduced phosphorylation PKCα ERK1/2, expression MMP9 MMP2, inhibited under SDF-1-stimulated conditions.CK inhibitor that inhibits regulating its downstream signaling molecules including PKCα, MMPs.