Antarctic Macroalgae <i>Palmaria decipiens</i> (Rhodophyta, Palmariales) Extracts Present Antioxidant and Antitumor Activity against Colorectal Cancer

• DOI: 10.4236/abc.2022.126022
• Publication Date: 2022-12-28T07:40:56Z
• Authors: Marina Della Gius...; Rafaele Frassini; Sidnei Moura; Fernando Joel Sca...; Sérgio Echeverrig...; Vanessa Sayuri Osaki; Franciane M. Pell...; Pio Colepicolo; João Antonio Pêga...; Janaina da Silva ...; Mariana Roesch-Ely
• Keywords: Propidium iodide; Ethidium bromide; Viability assay; Acridine orange

Despite therapeutic advances in recent decades, colorectal cancer is still the third most frequent neoplasm worldwide, with significant morbidity and mortality young middle-aged adults. Therefore, advance treatment options for patients who are afflicted tumor subtypes without effective therapies needed. Antarctica macroalgae substances-producing organisms important biological activities, which antitumor properties investigated, showing promising cytotoxic results. There no reports so far activity of Palmaria decipiens extracts against tumors. This study aims to evaluate effect P. extract from Antarctic on cell HCT-116 non-tumor HaCaT lines. The phenolic compounds present were identified by high performance liquid chromatography. antioxidant was determined DPPH radical inhibition method cytotoxicity evaluated through MTT assay. Cell death events using dual staining acridine orange/ethidium bromide flow cytometry. quantification presence three main among them kaempferol. metanolic showed within 72 h potential activity. results presented this point out an imbalance redox metabolism also a loss mitochondrial membrane integrity, likely inducing mechanisms after exposure extract. These could be observed penetration propidium iodide damage. indicate that interferes action cells, acting as agent.