New Amphiphilic Amino Acid Derivatives for Efficient DNA Transfection <i>in Vitro</i>

• DOI: 10.4236/aces.2017.72014
• Publication Date: 2017-04-11T08:11:23Z
• Authors: Lucía C. Peña; María F. Argarañá; María M. De Zan; Antonella Giorello; Sebastián Antuña; Claudio C. Prieto; Carolina M. I. Ve...; Diana M. Müller
• Keywords: CYSTEINE; https://purl.org/becyt/ford/1.4; https://purl.org/becyt/ford/1; 01 natural sciences; AMPHIPHILE; 3. Good health; 0104 chemical sciences

Nucleic acids-based therapies have recently developed as next-generation agents for treating and preventing viral infection, cancer, genetic disorders, but their use is still limited due to its relatively poor delivery into targeted cells. We designed synthesized new amphiphilic amino acid derivatives (cysteine-based) of low molecular weight, formed by the same pentapeptide (AG2: WWCOO) N-acylated, with different hydrophobic chains containing from 12 18 carbons, named AG2-Cn (N), which dimerize oxidation in presence pLenti-CMV-GFP Puro plasmid (P) respective gemini. determined transfection efficiency, critical micelle concentration, particle size, ζ-potential cytotoxicity obtained. found that all compounds were active DNA had greater ability transfect CHO-K1 In particular, AG2-C18 a promising carrier gene because it showed no activity was than or equal commercial actives currently used.