Infection of Laboratory-Colonized Anopheles darlingi Mosquitoes by Plasmodium vivax

• DOI: 10.4269/ajtmh.13-0708
• Publication Date: 2014-02-18T02:38:09Z
• Authors: Marta Moreno; Carlos Tong; Mitchel Guzmán; Raul Chuquiyauri; Alejandro Llanos-...; Hugo Rodriguez; Dionicia Gamboa; Stephan Meister; Elizabeth A. Winz...; Paula Maguina; Jan E. Conn; Joseph M. Vinetz
• Keywords: Vivax malaria; Plasmodium (life cycle)

Anopheles darlingi Root is the most important malaria vector in Amazonia region of South America. However, continuous propagation An. laboratory has been elusive, limiting entomological, genetic/genomic, and vector–pathogen interaction studies this mosquito species. Here, we report establishment an colony derived from wild-caught mosquitoes obtained northeastern Peruvian Amazon Iquitos Loreto Department. We show that numbers eggs, larvae, pupae, adults continue to rise at least F6 generation. Comparison feeding Plasmodium vivax ex vivo F4 F5 F1 generation showed comparable presence oocysts sporozoites, with corresponded blood-stage asexual parasitemia gametocytemia, confirming P. vectorial capacity colonized mosquitoes. These results provide new avenues for research on biology study – interactions.