Diagnosis of Plasmodium vivax by Loop-Mediated Isothermal Amplification in Febrile Patient Samples from Loreto, Perú

• DOI: 10.4269/ajtmh.20-0212
• Publication Date: 2020-08-04T07:02:24Z
• Authors: Oscar Nolasco; Beronica Infante; Juan Contreras-Ma...; Sandra Incardona; Xavier C. Ding; Dionicia Gamboa; Katherine Torres
• Keywords: Kappa; Vivax malaria; Diagnosis of malaria; Plasmodium (life cycle)

Plasmodium vivax is co-endemic with falciparum in Peru, and optimum management requires distinguishing these two species the blood of patients. For differential identification P. other spp., LoopampTM Malaria Pan Detection Kit combination Loopamp Pv (Eiken Chemical Co. Ltd., Tokyo, Japan) was used to evaluate 559 whole samples collected 2017 from febrile patients suspected malaria attending different health facilities Loreto region. The showed a sensitivity 87.7% (95% CI: 83.5-91.9) specificity 94.4% 91.9-96.9) good agreement PCR (Cohen's kappa 0.8266, 95% 0.7792-0.874). By comparison, similar (84.4%, 79.0-89.7) (92.4%, 89.7-95.0) substantial kappa: 0.7661, 0.7088-0.8234).