EVIDENCE FOR TRANSMISSION OF PLASMODIUM VIVAX AMONG A DUFFY ANTIGEN NEGATIVE POPULATION IN WESTERN KENYA

• DOI: 10.4269/ajtmh.2006.75.575
• Publication Date: 2018-09-01T06:18:05Z
• Authors: JEFFREY R. RYAN; JOSÉ A. STOUTE; JOSEPH AMON; RAYMOND F. DUNTON; RAMADHAN MTALIB; JOSEPH KOROS; BOAZ OWOUR; SHIRLEY LUCKHART; ROBERT A. WIRTZ; JOHN W. BARNWELL; RONALD ROSENBERG
• Keywords: Circumsporozoite protein; Merozoite surface protein

We present evidence that a parasite with characteristics of Plasmodium vivax is being transmitted among Duffy blood group-negative inhabitants Kenya. Thirty-two 4,901 Anopheles gambiae and An. funestus (0.65%) collected in Nyanza Province were ELISA positive for the P. circumsporozoite protein VK 247. All positives found late rainy season, when predominated, disproportionately many at single village. A specific sequence SSU rRNA gene was amplified from three six ELISA-positive mosquitoes. Erythrocytes 31 children, including 9 microscopically diagnosed as infected vivax, negative by flow cytometry Fy3 or Fy6 epitopes, which indicate group expression. DNA fragment C terminus merozoite surface 1 (MSP-1) four these children subsequently sequenced two.