One-Step Multiplex Polymerase Chain Reaction Assay for the Detection of Major Disease-Transmitting Mosquito Vectors in India

• DOI: 10.4269/ajtmh.24-0211
• Publication Date: 2024-11-26T14:27:47Z
• Authors: Mintu Karan; Sharmistha Paul; Supriya Nath; Bedanta Das; Sanhita Ghosh; Suman Karmakar; Pritam Mandal; Biplab Bhowmik; Piyoosh Kumar Singh; Rajnikant Dixit; Chiranjib Pal
• Keywords: Multiplex

Mosquitoes are important vectors that transmit viral, protozoan, and helminthic diseases across the world. Climate change unplanned urbanization accelerating spread of these diseases. Controlling vector-borne can be performed most effectively through vector control. Inadequate knowledge bionomics is an impediment lead to inappropriate control efforts. However, conventional methods identification based on morphological differences, demand a significant amount time specific skills, often misleading. An efficient affordable solution needed quickly accurately identify pooled samples from vast geographical territories. To ensure correct distorted or in India, set definitive steps required, including construction unique primers standardization one-step assay second internal transcribed spacer gene ribosomal DNA. We have successfully developed confirmed highly multiplex reverse transcriptase polymerase chain reaction for accurate major mosquito vectors, especially cases both adult larval forms Anopheles sp., Aedes Culex sp. Hence, specificity, universality, uniqueness could serve as critical tool rapid one-reaction mosquitoes mosquito-borne disease outbreaks public health emergencies.