Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines

• DOI: 10.4308/hjb.30.3.522-531
• Publication Date: 2023-03-06T07:54:39Z
• Authors: Omat Rachmat Hasb...; Ilma Fiddiyanti; Dewi Ratih Handayani; Endang Sutedja; Darmadji Ismono; Nucki Nursjamsi H...; Wahyu Widowati; Ervi Afifah; Hanna Sari Widya ...; Rizal Rizal; Firman Alamsyah; Warsito P. Taruno
• Keywords: QH301-705.5; Biology (General); 3. Good health

Osteosarcoma is a highly malignant primary tumor on bone that mainly attacks children and young adolescents. Until now, osteosarcoma therapy still combines some high costs invasive modalities may give side effects, such as pain nausea. Our previous studies suggested non-contact electric field has anti-proliferative effect breast cancer cells, in vitro vivo. In this study, we were interested studying alternating current effects cells progression well its potential cytotoxic effects. MG-63 human cultured treated with 200 kHz for 6 days. Several genes of interest including p53, p21, MDM2, caspase-3, caspase-8, caspase-9 analyzed using real-time qPCR method. Apoptotic index was measured flow-cytometry assay. Apoptosis observed through p53-independent p21 pathway (p = 0.011). Cells undergoing apoptosis internal pathways shown by the increase caspase-3 0.015) 0.001) levels, but not caspase-8 0.080). This treatment successfully reduced number living 14.7% 0.000) increased cell death up to 4.26% 0.055). markedly 16% 0.001). exposure can disrupt disruption normal cycle via induction apoptosis.