Determination of cordycepin content of <i>Cordyceps militaris</i> recombinant rice by high performance liquid chromatography

• DOI: 10.4314/tjpr.v15i10.23
• Publication Date: 2016-11-18T01:50:44Z
• Authors: Xuesong Wang; Fang Liu; Fang Li; Hongyan Cai; Wei Sun; Xuan Chen; Hong Gao; Wangyang Shen
• Keywords: Cordycepin; Cordyceps militaris; Repeatability

Purpose: To assess the suitability of high performance liquid chromatography (HPLC) for determination cordycepin content Cordyceps militaris recombinant riceMethods: rice was made by mixing brown with artificial Chinese caterpillar fungus culture medium powder using twin-screw extrusion technology. Cordycepin determined reversed-phase HPLC water:acetonitrile (95:5, v/v) as mobile phase, detection wavelength 260 nm, and flow rate 1.0 mL/min.Results: contents showed good linearity in range 1 - 50.0 μg/mL (r2 = 0.9996), while recovery ranged from 103.2 to 109.9 %. Relative standard deviation (RSD), precision repeatability RSD 2.38, 0.76 1.46 %, respectively.Conclusion: The method is simple, fast, accurate reproducible. It suitable rice.Keywords: Recombinant rice, Cordycepin, fungus, Aweto,