High iNOS and IL-1β immunoreactivity are features of colitis-associated colorectal cancer tumors, but fail to predict 5-year survival

• DOI: 10.48101/ujms.v128.10241
• Publication Date: 2024-01-02T15:11:06Z
• Authors: Kajsa Björner; Wei-Na Chen; Venkata Ram Ganna...; Fredrik Axling; Miklos Gulyas; Mohammad Abdul Halim; Dominic-Luc Webb; Per M. Hellström
• Keywords: Intestinal mucosa; Pathogenesis

Background: Inflammatory bowel disease (IBD; mainly ulcerative colitis and Crohn’s disease) is associated with the development of colorectal cancer (CRC) referred to as colitis-associated (CAC). In inflammatory flares IBD, production luminal nitric oxide (NO) increases due increased inducible synthase (iNOS) activity in inflamed tissue. It believed that iNOS parallels pro-inflammatory interleukin-1β (IL-1β). How these biomarkers relate CAC pathogenesis or survival unknown.
 Aim: The primary aim this study was investigate IL-1β immunoreactivity tumors comparison CRC normal colonic mucosa, secondary determine if correlates 5-year CAC.
 Methods: Immunohistochemistry performed on tissue sections follows: (n = 59); sporadic (sCRC) 12); mucosa >2 cm outside sCRC margin (normal mucosa) 22); paracancerous IBD (pIBD) 12). expression quantified separately for epithelium stroma. Data were evaluated using Mann-Whitney U-test log-rank test Kaplan-Meier curves. Results compared online mRNA databases.
 Results: Immunoreactivity occurred predominantly epithelial cells lesser extent Compared (P < 0.01) 0.005) higher epithelium. stroma, lower than 0.001), whereas 0.05). differences among patients failed correlate survival. These findings supported by Conclusion: Consistent high NO there more epithelium, albeit not This difference exists both intervention arginine chemotherapy straightforward.