Circulating Aneuploid Cells Detected in the Blood of Patients with Infectious Lung Diseases

• DOI: 10.5090/kjtcs.2017.50.2.126
• Publication Date: 2017-03-31T03:35:34Z
• Authors: Hongsun Kim; Jong Ho Cho; Chung-Hee Sonn; Jae-Won Kim; Yul Choi; P.N.; Jinseon Lee; Jhingook Kim
• Keywords: Immunofluorescence; Circulating tumor cell

The identification of circulating tumor cells (CTCs) is clinically important for diagnosing cancer. We have previously developed a size-based filtration platform followed by epithelial cell adhesion molecule immunofluorescence staining detecting CTCs. To characterize CTCs independently surface protein expression, we incorporated chromosomal fluorescence in situ hybridization (FISH) assay to detect abnormal copy numbers chromosomes collected from peripheral blood samples the platform. Aneuploid were detected patients with lung Unexpectedly, aneuploid also control group, which consisted benign diseases, such as empyema necessitatis and non-tuberculous mycobacterial disease. These findings suggest that abnormalities are observed not only cells, but infectious diseases. Thus, our present new considerations bring into light possibility false positives when using FISH cancer diagnosis.