The effect of horse placenta extract on human peripheral blood mononuclear cells

• DOI: 10.51248/.v40i2.65
• Publication Date: 2020-11-11
• Authors: Ildar Fakhradiyev; B.O. Minbayev; Aigul Tazhiyeva; A.M. Zhukembayeva; Z.Z. Batagoyeva; Baimakhan Tanabayev; Yerlan Kuandykov; Naylia Ussebayeva
• Keywords: Propidium iodide

Introduction and Aim:Placental extracts due to the unique biological properties have been widely utilized in various fields of medicine. The objective study was investigate effect horse placenta extract on human peripheral blood mononuclear cells (PBMCs). Materials Methods:The obtainedfrom mares. PBMCs were isolated from venous samples healthy volunteers. To assess toxic (in final protein concentrations 1500, 750, 375, 187.5, 93.75, 46.88 ?g/ml) an MTT test employed. As a control, cultures untreated with used. cytotoxicity examined by using propidium iodide (PI). A CFSE evaluate T-cell proliferation. levels reactive oxygen species (ROS) also evaluated. An enzyme-linked immune-sorbent assay used determine level interleukin secretion. Results:According results assay, observed at concentration 1500 ?g/ml 750 ?g/ml. PI showedthat solution had no after 72 h (concentration range 46.9 ?g/ml). However, hours exposure 3250 ?g/ml, where cytotoxicitywas 4.6 12.0%, respectively (p ? 0.05). 2-hour incubation 375 led significant decrease ROS In addition, proliferation considerably reduced extract's compared control detected increase production IL-4 PBMC (24-hour incubation) dependent extract. Conclusion:The indicate that does not possess towards PBMCs. Moreover, we immune-modulating extract. Keywords:Horse extract; cytotoxicity; immune modulation.