Selection of Genetic Markers to Determine Diversity in Theileria annulata Populations after Recombination

• DOI: 10.5152/tpd.2017.4970
• Publication Date: 2017-05-05T14:07:21Z
• Authors: Huseyin Bilgin Bi...; Ahmet Hakan Unlu; Ayca Aksulu; Serkan Bakirci; Selin Hacilarlioglu; Hasan Eren; William Weir; Tulin Karagenc
• Keywords: Minisatellite; DNA profiling

Selecting polymorphic mini- and microsatellite markers to determine genetic diversity chromosomal regions exhibiting elevated rates of recombination in Theileria annulata populations after recombination.The Unipro UGENE software was used select markers. A score at which 10 times more tandem repeats (TRs) were identified the real DNA sequence than those scrambled sequences T. as cutoff. TRs containing minimum three nucleotides length for six minisatellite having a repeat motif identity between 96%-100% with unit size repeated screened through whole genome using suffix array algorithm.A total 359 minisatellites 8973 microsatellites identified. one by genome; representing single region suitable primer design selected based on their localization chromosomes, (>96%), (<1500 bp). The primers amplify candidates designed, each check 27 different isolates annulata.In present study, 13 located chromosomes population annulata.