Detection of microbiota in the vineyards of the Tokaj wine region

• DOI: 10.5219/1246
• Publication Date: 2019-12-13T10:31:55Z
• Authors: Ivana Regecová; Slavomí­r Marcinčák; Jozef Nagy; Peter Popelka; Boris Semjon; Pavlina Jevinová; Monika Pipová; Martin Král; Marián Kovalčí­k
• Keywords: Vineyard; Candida parapsilosis; Most probable number

Tokaj is an important Central European wine-growing area with controlled planting and authorized varieties of white vines. This has a specific microflora composition which changes based on its climate dependence, as well during the fermentation process wine production. Therefore, aim this study was, by culture examination samples, to detect microbiota soil, leaves, berries must from two vineyards Slovak part Tokaj. The highest total viable count (5.60 ±0.01 log cfu.g-1) yeast mould (4.32 in soil samples were recorded vineyard Berecký. (6.71 was confirmed originating Čierna Hora. When determining count, numbers (4.15 cfu.mL-1). Lactic acid bacteria collected both vineyards, only very low numbers. Overall, statistically significant differences (p <0.001) detected comparing taken Berecký Hora vineyards. characterisation identification carried out using ITS-PCR-RFLP methods. analysis presence yeasts Saccharomyces cerevisiae, Zygosaccharomyces bailii, rouxii, Candida parapsilosis tenuis their subsequent transfer at varying percentages.