Efficacy of ozone therapy in the treatment of frozen shoulder in rats: An experimental study

• DOI: 10.52312/jdrs.2025.2055
• Publication Date: 2025-01-20T13:25:32Z
• Authors: Ekin Kaya Simsek; Duygu Turkbey Simsek; Deniz Mert Ayan; Coskun Araz; Bahtiyar Haberal
• Keywords: Ozone Therapy; Frozen shoulder

This study aims to explore the therapeutic effects of medical ozone therapy on frozen shoulder (FS) model and compares it with traditional corticosteroid treatments in rats. A total 30 Sprague-Dawley rats aged 18 20 months weighing between 400 450 g were included study. The randomly divided into three equal groups: a control group (C, n=10, FS only), treatment (CST, + intraarticular 0.5 mg/kg betamethasone), an (OT, 1 ozone). Frozen was induced via surgical immobilization, administered intraarticularly. Outcomes measured through histopathological functional assessments. CST OT significantly reduced inflammation (p<0.001), capillary proliferation fibroblastic (p=0.002), collagen type 3 staining (p=0.022), mean capsular thickness while improving range motion all directions compared group. Ozone showed comparable reduction fibrosis improvement joint mobility (p=0.001). effectively reduces improves rat model, presenting promising alternative corticosteroids. However, further studies are still needed elucidate molecular mechanisms optimize protocols, underscoring potential for future discoveries this area.