Sphingosine-1-phosphate induces Ca²⁺ mobilization via TRPC6 channels in SH-SY5Y cells and hippocampal neurons

• DOI: 10.52396/justc-2022-0014
• Publication Date: 2022-09-30T06:00:43Z
• Authors: Haotian Wu; Bingqian Lin; Canjun Li; Wenping Zeng; Lili Qu; Chunlei Cang
• Keywords: TRPC6; Sphingolipid

Sphingosine-1-phosphate (S1P) is a widely expressed biologically active sphingolipid that plays an important role in cell differentiation, migration, proliferation, metabolism and apoptosis. S1P activates various signaling pathways, some of which evoke Ca<sup>2+</sup> signals the cytosol. Few studies have focused on mechanism by evokes neurons. Here, we show global SH-SY5Y cells hippocampal Removal extracellular calcium largely abolished S1P-induced increase intracellular Ca<sup>2+</sup>, suggesting influx major contributor to this process. Moreover, found mobilization independent G protein-coupled receptors. The TRPC6 inhibitor SAR7334 suppressed signals, indicating channel acts as downstream effector S1P. Using patch-clamp recording, showed currents. Two Src tyrosine kinase inhibitors, Src-I1 PP2, dramatically inhibited activation Taken together, our data suggest channels Src-dependent way induce