CLINICAL STUDY OF GENE EXPRESSION PROFILE AND SOME OF THE MOST COMMON CHROMOSOMAL ABERRATIONS IN UNTREATED B-CHRONIC LYMPHOCYTIC LEUKAEMIA PATIENTS

• DOI: 10.5272/jimab.2020261.2905
• Publication Date: 2020-02-12T11:55:33Z
• Authors: Vanya Slavcheva; Georgi Balatzenko; ; Svetlana Angelova; ; Emilia Naseva; ; Tzvetan Lukanov; ; Nikolai Tzvetkov
• Keywords: Medicine (General); del13q14; lipoprotein lipase; RK1-715; lymphocytic leukaemia; 3. Good health; 03 medical and health sciences; R5-920; 0302 clinical medicine; adam29; Dentistry; prognosis

Aim: Chronic lymphocytic leukaemia (CLL) is one of the most common leukaemias in adults. Unlike myeloid and acute lymphoblastic leukaemia, treatment can be delayed after diagnosis. Exploration of the unique biology of the disease has led to the identification of more than 35 prognostic factors reported in the literature. In practice, however, the number of validated clinical and biological prognostic factors is significantly smaller. The purposes of our study are: to investigate the distribution of untreated CLL- patients according to Binet staging as well as to analyse the frequency of one of the most common chromosomal aberrations in the group. To study the correlation between mutational status (determined by evaluation of lipoprotein lipase/ ADAM29 ratio), and the identified chromosomal aberrations in the group of 48 untreated patients. aterials and methods: We studied 48 patients with a confirmed flowcytometric diagnosis B-CLL, using the 2008 IWCLL-NCI criteria. The interphase fluorescence in situ hybridization (FISH) was applied to determine the chromosomal abnormalities. Analysis of mutational status was performed as described by Pablo Oppezzo with slight modification. Documentary and statistical methods were used for data processing by Fisher`s Exact test. Conclusion: We found that a large proportion of patients were diagnosed with early-stage A of the disease. In most untreated CLL- patients isolated del13q was detected. Statistically, a significant association was found between del13q, del11q, del17p / p53 and mutational status.