Abdominal Hernia Repair with Bovine Pericardium Seeded with Mesenchymal Stem Cells in Wistar Rats

• DOI: 10.5281/zenodo.7044894
• Publication Date: 2022-09-02
• Authors: Silvana Bellini V...; Janete Maria Volp...; Lanucha Fidelis d...; Carolina Cavaliér...; Ana Helena da Ros...; Herbert Martins G...; Luíse Meurer; Rodrigo Rodrigues...; Karina Magano Gui...; Elizabeth Obino C...; Emerson Antônio C...
• Keywords: 03 medical and health sciences; 0302 clinical medicine; 3. Good health

Background: Biological membranes demonstrate superiority over synthetic ones for its biocompatibility and strength in the reduction of abdominal hernias. Recents tissue engineering researches add mesenchymal stem cells to biological with purpose obtaining additional cellular proliferation consequent muscle regeneration, using as scaffolds. This article aimed study infl uence regeneration hernias, reduced membranes. Materials, Methods & Results: Adult Wistar rats underwent hernia-inducing. They were divided into two groups form treatment hernia: associated or only After macro microscopic reviews carried out days seven, 14 60 postoperatively. Preparation bovine pericardium glycerin 98% presented effi ciency decellularization conservation, maintaining avoiding bacterial growth. The obtained from bone marrow adult rats, had capacity proliferation. majority was positive expression surface antigens CD44, CD29 CD99 negative CD 34. In differentiation trials, same able differentiate adipocytes osteocytes. With 24 h co-cultivating adhesion observed. There no foreign body reaction contamination surgical wounds there intense postoperative neovascularization on seven days. All animals omentum adherence, but adherence other organs.There statistically difference different times macroscopic assessment: deposition fi brous tissue, implant integration. occurred evaluations between groups. immediate later repair responses treatment. Discussion: use animal model satisfactory, being suitable procedures assessments cavity. results late corroborate idea that is induction models surgery surgeries time interval two, suggesting need methodologies simulate hernias chronicity. used classifi ed cells, because it met all criteria Mesenchymal Tissue Stem Cell Committee International Society Celullar Therapy. conserved demonstrated biocompatibility, rejection necrosis, infection exacerbated ammation. However not methodological latest experiments about can leads conclusion attached insuffi cient number obtain desired result. These suggest new research studies methods association cells.