Identification of specific carbonic anhydrase inhibitors via in situ click chemistry, phage-display and synthetic peptide libraries: comparison of the methods and structural study

• DOI: 10.5281/zenodo.7260582
• Publication Date: 2023-01-01
• Authors: Michael Kugler; Martin Hadzima; Rastislav Dzijak; Robert Rampmaier; Pavel Srb; Lukáš Vrzal; Zdeněk Voburka; Pavel Majer; Pavlína Řezáčová; Milan Vrabel
• Keywords: 0301 basic medicine; 03 medical and health sciences; combinatorial libraries; generation; Carbonic anhydrase, in situ click, phage display, libraries, OBOC; fragment-based discovery

Here we show how different selection methods can be used for the discovery of a selective and potent carbonic anhydrase inhibitors. X-Ray and NMR structural studies were used to reveal the key binding interactions of the inhibitor with the enzyme.