Production and characterization of antimicrobial compound produced by Streptomyces atrovirens H33

• DOI: 10.5281/zenodo.7269694
• Publication Date: 2022-11-01
• Authors: Alaa-ElDin M S Hosny; Donia H Sheir; Ahmed I El-Diwany; Nayera A M Abdelw...; Adyary Fallarero; Pia M Vuorela
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; 16S rRNA, bioreactor; cytotoxicity, HPLC MS/MS, Streptomyces atrovirens H33.; 6. Clean water; 3. Good health

ABSTRACT Streptomyces atrovirens was isolated from soil samples in Egypt and showed broad spectrum antimicrobial activity. It was identified as Streptomyces atrovirens (strain H33) based upon16S rRNA gene sequencing. It was deposited in the GenBank database under accession number of KJ435269. Streptomyces atrovirens (strain H33)was cultivated by submerged fermentation bioreactor to produce the antimicrobialmetabolites. Purification and identification of antimicrobial compound was carried out. The antimicrobial compound exhibited low cytotoxic effect on human epithelial HL cells. Key words: 16S rRNA, bioreactor; cytotoxicity, HPLC MS/MS, Streptomyces atrovirens H33. REFERENCES Bauer, A.W., Kirby, W.M. M., Sherris, J.C., and Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol.45, 493-496. Berdy, J. (2005). Bioactive microbial metabolites. J. Antibiot. (Tokyo), 58, 1-26. Cho, J. Y. and Kim, M. S. (2012) Antibacterial benzaldehydesproduced by seaweed-derived Streptomyces atrovirens PK288-21. Fish. Sci., 78, 1065-1073. Elibol, M., Ulgen, K., Kamarulzaman, K., andMavituna, F. (1995). Effect of inoculum type on actinorhodin production by Streptomyces coelicolor. Biotechnol. Lett.,17, 579-582. Felsenstien, J. (1985). Confidence limits on phylogenies: an approach usingthe bootstrap.Evolution, 39, 783-791. Garrod, L. P. and Waterworth, P. M. (1971). A study of antibiotic sensitivity testing with proposals for simple uniform methods. J. Clin. Pathol.,24, 779-789. Hobbs, G., Frazer, C.M., Gardner, D.J.C., Cullum, J.A., and Oliver, S.G. (1989). Dispersed growth of Streptomyces in liquid culture. Appl. Microbiol. Biotechnol.,31, 272-277. Hosny, A.M. S., Sheir, D. H., Abdelallah,N. A., Amin, D. H. M., El-Diwany,A. I., Abdelwahed, N.A. M., El-Beih,A. A., Fallarero,A., and Vuorela,P. M. (2015). Isolation ofStreptomyces sp. producing antifungal agent. Vol. 4 (2) April-June, Curr.Sci. Int. (In press). Karlsson, D., Fallarero. A., Brunhofer, G., Mayer, C., Prakash, O.et al. (2012). The exploration of thienothiazines as selective butyrylcholinesterase inhibitors. European Journal of Pharmaceutical Sciences, 47,190-205. Kim,K-J., Kim,M-A., and Jung,J-H. (2008). Antitumor and antioxidant activity of protocatechualdehyde produced from Streptomyces lincolnensis M-20. Arch. Pharm. Res., 31, (12), 1572-1577. Lane, D., Pace, B., Olsen, G., Stahl, D., Sogin , M. et al. (1985). Rapid determination of 16S ribosomal RNA sequence for phylogenetic analyses. Proc. Natl. Acad. Sci.U. S. A., 82, 6955 –6959. Locatelli, L., Tarnawski, S., Hamelin, J., Rossi,P., Aragno, M. et al. (2002). Specific PCR amplification for the genus Pseudomonas targeting the 3 half of 16S rDNA and the whole 16S- 23S rDNA spacer. Syst. Appl. Microbiol.,25, 220-227. Mann, J. (2001). Natural products as immunosuppressive agents. Natural Product Reports, 18, 417-430. Olano,C., Moss, S. J., Braña, A. F., SheridanR. M., MathV. et al. (2004). Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tü4055: insights into nitrile formation. Mol. Microbiol.,52 (6), 1745–1756. Oldfield, C., Wood, N. T., Gilbert, S. C., Murray, F. D., and Faure, F. R. (1998). Desulphurisation of benzothiophene and dibenzothiophene by actinomycete organisms belonging to the genus Rhodococcus, and related taxa. Antonie Van Leeuwenhoek, 74,119-132. Pranitha, G. Krishna and M.A Singara Charya (2014). Evaluation of antibacterial and anifungal activity of fruiting Body extracts of trametes versicolar. Biolife. 2(4):1181-1184. Raintala, H., Nvalainen, A., Ronka, E., and Suutari, M. (2001). PCR primers targeting the 16S rRNA gene for the specific detection of Streptomyces. Mol. Cell. Probes,15, 337-347. Raja A. and Prabakarana P. (2011). Actinomycetes and drug-An overview. Am. J. of Dru. Dis. and Dev., 1, 75-84. Saito, N. and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4, 406-425. Shaaban, K.A., Shepherd, M.D., Ahmed, T.A., Nybo, S.E., Leggas, M. et al. (2012). Pyramidamycins A-D and 3-Hydroxyquinoline-2-carboxamide; Cytotoxic Benzamides from Streptomyces sp. DGC1.J.Antibiot. (Tokyo),65(12): 615-622. Shirling, E.B. and Gottlieb, D. (1966). Methods for characterization of Streptomyces sp. Int. J. Syst. Bacteriol.,16, 313-340. Su, W. W. (2006). Bioreactor engineering for recombinant protein production using plant cell suspension culture. In: Plant Tissue Culture Engineering, S. Dutta Gupta and Y. Ibaraki (Eds), Springer, Printed in the Netherlands, pp. 135-159. Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S.(2013). MEGA6: Molecularevolutionary genetics analysis version 6.0. Mol. Biol. Evol., 28(10), 2731-2739. Vandamme, E.J. (1983). Peptide antibiotic production through immobilized biocatalyst technology. Enzyme Microb. Technol.,5, 403–416