A Comparative Investigation of Antimicrobial Activities of Proteins Derived From Anodonta Rubens

• DOI: 10.5281/zenodo.890190
• Publication Date: 2015-04-13
• Authors: Essam M. Ahmed
• Keywords: 0301 basic medicine; 03 medical and health sciences; Anodonta rubens, antiviral and antimicrobial. Protein fractions

Protein extracted from Anodonta rubens (Mollusca) was evaluated to investigate in vitro the anti-microbial and the antiviral activities. The crude protein was fractionated by precipitation by different methods. Different measures were determined such as inhibition zone (mm), MIC (minimum inhibitory concentration), EC50. The toxicity of the most active fractions was measured by the XTT assay then CC50 and selective index value were calculated. Amino acid analysis was performed for the purified fraction. Some of the tested protein showed activities with various degrees. The most potential antiviral activity, about 96%, was achieved in case of 75-90% saturation of Polyethylene glycol of molecular weight 4000 (PEG 4000). The protein fractions showed low toxicity. The fraction is rich in essential amino acids, alanine, arginine, glutamine, glycine and phenylalanine. To the best of our knowledge, no similar works were reported with this organism and these compounds..