Chitosan nanoparticles as a new technique in gene transformation into different plants tissues

• DOI: 10.53365/nrfhh/144414
• Publication Date: 2022-01-07T00:18:18Z
• Authors: Eman Tawfik; Mohamed Ahmed
• Keywords: 0303 health sciences; 03 medical and health sciences

The utilization of chitosan nanoparticles is a novel technique for gene transformation into plant tissues. It takes few minutes to transform plant. UidA was detected in <i>Escherichia coli</i> (K12 strain) using polymerase chain reaction analysis by UidA-specific primers. transformed the explants two different species (<i>Solanum tuberosum</i> and <i>Paulownia tomentosa</i>). These plants have natures as crop woody respectively. Therefore, they abilities express gene. expressed blue color tissues due formation expression GUS enzyme. morphologically color; molecular PCR. Chitosan were characterized UV/Visible spectroscope photographing with transmission electron microscope (TEM). As result this research, it suggested that be used Because safe, quick, inexpensive, well biocompatible biodegradable.